The Korea  Cancer Coleotherapy 

Huh JE. Kang KS. Ahn KS. Kim DH. Saiki I. Kim SH. Mylabris phalerlata induces apoptosis by caspase activation following cytochrome c release and Bid cleavage. Life Sciences. 73(17):2249-62, 2003 Sep 12.

Institution
Department of Oncology, Graduate School of East-West Medicine, KyungHee University, 1 Seochunri, Yongin 449-701, South Korea.

Abstract
Mylabris phalerata (MP) is an insect that has been used for the treatment of cancer in oriental medicine. In the present study, the butanol (BuOH) fraction of MP (BFMP) was examined to determine whether it can exert anti-cancer activity through an apoptotic pathway with little toxicity. BFMP was found to have a specific cytotoxic effect on human monocytic leukemic U937 cells (IC(50) = 140 microg/ml) rather than on peripheral blood mononuclear lymphocytes (PBML, IC(50) = over 500 microg/ml). BFMP also induced the morphological changes of apoptosis, such as chromatin condensation, cell shrinking and DNA fragmentation at a concentration of 31.25 microg/ml. In addition, BFMP significantly increased the portion of apoptotic annexin-V positive cells in a dose-dependent manner, and effectively activated caspases (cysteine aspartase) cascade involving caspases 8, 9 and 3. BFMP also effectively cleaved Bid, a death agonist member of the Bcl-2 family and (poly(ADP-ribose)polymerase) (PARP) and induced the subsequent release of cytochrome c from mitochondria into the cytosol. However, it did not affect Bcl-2 and Bax expression. Taken together, these data suggest that the BuOH extract of Mylabris phalerata can induce apoptosis in U937 cells by caspase cascade activation in conjunction with cytochrome c release, induced by a product of Bid. Therefore, we conclude that BFMP has anti-cancer activity, which is achieved through apoptosis and is associated with little toxicity.

 Special Thanks José Conseco ( Lima Perú )

La Coleoterapia y el Cáncer en Korea  

Estamos traduciendo este artículo ( 18 de Abril 2005 ) 

utilizan otro coleóptero el ( Mylabris phalerata Pallas ) 
ya lo tienen procesado en un medicamento.