Korea Cancer Coleotherapy
JE. Kang KS. Ahn KS. Kim DH. Saiki I. Kim SH. Mylabris
phalerlata induces apoptosis by caspase activation following
cytochrome c release and Bid cleavage. Life Sciences.
73(17):2249-62, 2003 Sep 12.
Department of Oncology, Graduate School of East-West Medicine,
KyungHee University, 1 Seochunri, Yongin 449-701, South Korea.
Mylabris phalerata (MP) is an insect that has been used for
the treatment of cancer in oriental medicine. In the present
study, the butanol (BuOH) fraction of MP (BFMP) was examined
to determine whether it can exert anti-cancer activity through
an apoptotic pathway with little toxicity. BFMP was found to
have a specific cytotoxic effect on human monocytic leukemic
U937 cells (IC(50) = 140 microg/ml) rather than on peripheral
blood mononuclear lymphocytes (PBML, IC(50) = over 500 microg/ml).
BFMP also induced the morphological changes of apoptosis, such
as chromatin condensation, cell shrinking and DNA
fragmentation at a concentration of 31.25 microg/ml. In
addition, BFMP significantly increased the portion of
apoptotic annexin-V positive cells in a dose-dependent manner,
and effectively activated caspases (cysteine aspartase)
cascade involving caspases 8, 9 and 3. BFMP also effectively
cleaved Bid, a death agonist member of the Bcl-2 family and (poly(ADP-ribose)polymerase)
(PARP) and induced the subsequent release of cytochrome c from
mitochondria into the cytosol. However, it did not affect Bcl-2
and Bax expression. Taken together, these data suggest that
the BuOH extract of Mylabris phalerata can induce apoptosis in
U937 cells by caspase cascade activation in conjunction with
cytochrome c release, induced by a product of Bid. Therefore,
we conclude that BFMP has anti-cancer activity, which is
achieved through apoptosis and is associated with little
Thanks José Conseco ( Lima Perú )
Coleoterapia y el Cáncer en Korea
traduciendo este artículo ( 18 de Abril 2005 )
otro coleóptero el ( Mylabris
lo tienen procesado en un medicamento.